Author : OECD
Publisher : OECD Publishing
Page : 6 pages
File Size : 24,10 MB
Release : 1986-10-23
Category :
ISBN : 9264071423
This assay may be used to measure mitotic recombination (gene conversion or crossingover) in yeast, a eukaryotic micro-organism. Crossing-over is generally assayed by the production of recessive homozygous colonies or sectors produced in a ...
Author : OECD
Publisher : OECD Publishing
Page : 5 pages
File Size : 45,88 MB
Release : 1986-10-23
Category :
ISBN : 9264071407
This assay may be used to measure gene mutation in yeast, a eukaryotic micro-organism. Strains of Saccharomyces cerevisiae have been developed which detect forward or reverse mutations. A variety of haploid and diploid strains of the yeast can be ...
Author : OECD
Publisher : OECD Publishing
Page : 6 pages
File Size : 12,48 MB
Release : 1986-10-23
Category :
ISBN : 9264071504
The mouse heritable translocation test detects structural and numerical chromosome changes in mammalian germ cells as recovered in first generation progeny. The types of chromosome changes detected in this test system are reciprocal translocations ...
Author :
Publisher :
Page : pages
File Size : 41,77 MB
Release : 1986
Category :
ISBN :
Author : OECD
Publisher : OECD Publishing
Page : 7 pages
File Size : 26,22 MB
Release : 1986-10-23
Category :
ISBN : 926407144X
The Test Guideline for Unscheduled DNA Synthesis (UDS) in mammalian cells in vitro describes procedures utilizing primary cultures, human lymphocytes or established cell lines, to detect DNA repair synthesis after excision and removal of a stretch ...
Author : OECD
Publisher : OECD Publishing
Page : 4 pages
File Size : 35,25 MB
Release : 1986-10-23
Category :
ISBN : 9264071482
The mouse spot test detects presumed somatic mutations in fetal cells following transplacental absorption of the test substance. This is an in vivo test in mice in which developing embryos are exposed to a chemical (solid, liquid, vapour or ...
Author : OECD
Publisher : OECD Publishing
Page : 5 pages
File Size : 37,41 MB
Release : 1986-10-23
Category :
ISBN : 9264071385
The sister chromatid exchange (SCE) assay is a short-term test for the detection of reciprocal exchanges of DNA between two sister chromatids of a duplicating chromosome. Detection of SCEs requires some means of differentially labeling sister ...
Author : OECD
Publisher : OECD Publishing
Page : 29 pages
File Size : 49,32 MB
Release : 2022-06-30
Category :
ISBN : 9264203907
This Test Guideline describes an in vivo assay that detects chemicals that may induce gene mutations in somatic and germ cells. In this assay, transgenic rats or mice that contain multiple copies of chromosomally integrated plasmid or phage shuttle vectors are used. The transgenes contain reporter genes for the detection of various types of mutations induced by test chemicals.
Author : OECD
Publisher : OECD Publishing
Page : 6 pages
File Size : 23,22 MB
Release : 1984-04-04
Category :
ISBN : 9264071342
Mutations in the X-chromosome of Drosophila melanogaster are phenotypically expressed in males carrying the mutant gene. When the mutation is lethal in the hemizygous condition, its presence is inferred from the absence of one class of male ...
Author : OECD
Publisher : OECD Publishing
Page : 37 pages
File Size : 17,66 MB
Release : 2022-06-30
Category :
ISBN : 9264366067
This Test Guideline describes an in vivo erythrocyte Pig-a gene mutation assay (hereafter called the Pig-a assay) which uses an endogenous mammalian gene, the phosphatidylinositol glycan class A gene (Pig-a), as a reporter of somatic-cell gene mutation. In vivo gene mutation tests, such as the Pig-a assay, are especially relevant for assessing mutagenicity because physiological factors, such as absorption of the test chemical from the site of exposure, distribution of the test chemical throughout the test system via systemic circulation, and in vivo metabolism and DNA repair processes, all contribute to the mutagenic responses.
