Genetic and Agro-Morphological Diversity and Genotype by Environment Interaction of Yield and Nutritional Quality Traits in Pearl Millet Germplasm from Sudan


Book Description

Due to its adaptation to extremely harsh environments, pearl millet (Pennisetum glaucum (L) R. Br.) is one of the most important staple cereals cultivated in the arid and semi-arid tropics of Asia and Africa. In these regions pearl millet is mainly produced under low-input, rainfed conditions in traditional farming systems, where several constrains are affecting pearl millet production, such as inter-annual rainfall variability, pests, diseases, Striga and use of low yielding varieties. The majority of the human population in these regions is affected by nutritional deficiencies of essential minerals causing micronutrient malnutrition (so-called ‘hidden hunger’). Plant genetic resources provide basic materials for selection and improvement of crop productivity through the optimal design of breeding programs to meet food security needs of the world’s rapidly rising population. The advent of PCR-based molecular markers such as simple sequence repeats (SSRs) has created an opportunity for fine-scale genetic characterization of germplasm collections. Understanding the structure of diversity and the identification of distinct materials with complementary traits for recombination by crossing provides the foundation for effective and sustained pearl millet population breeding and synthetic and hybrid development, based on the concept of heterotic groups in this allogamous crop.
















The Ghanaian Pearl Millet [Pennisetum Glaucum, (L), R.Br] Landrace


Book Description

Germplasm (store of genetic information) characterisation can be carried out by means of morphological, biochemical, or molecular analysis. Molecular analysis using genomic DNA is reliable since it can be carried out at any developmental state of plant and offers the opportunity to efficiently compare all accessions. The Simple Sequence Repeats (SSR) markers used have demonstrated to be very informative in studying relationships in closely related plant species as well as readily detecting co-dominant inheritance and exhibiting a high level of polymorphism per loci. 126 Pearl millet accessions were collected from Northern Ghana, home to Pearl millet production in Ghana. These were grouped into Early, Medium and Late maturing classes. 36 Pearl millet SSR markers were used to genotype 119 of the accessions which revealed an average of 8.8 alleles per locus (range of 3 - 20). The results obtained indicate high polymorphism in the accessions. The sizes of alleles ranged from 98bp to 377bp. A combination of molecular and morpho-agronomic data resulted in 30 accessions selected as core to serve as trait-specific and local gene source for enhanced Pearl millet breeding work in Ghana.







The Pearl Millet Genome


Book Description