Somatic Embryogenesis: Fundamental Aspects and Applications


Book Description

This book discusses basic and applied aspects of somatic embryogenesis, one of the most powerful tools in plant biotechnology. It is divided into three parts; Part I includes topics such as the history of this research field, how differentiated plant cells can (re)acquire totipotency, molecular features, as well as the epigenetics and proteomics of somatic embryogenesis. Part II covers the somatic embryogenesis of different crops, such as Agave spp. maize, Cocos nucifera, Bixa orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and Arabidopsis thaliana. Various applications, like scale-up propagation and genetic engineering are discussed in detail in Part III. The book will appeal to plant scientists, plant breeders and experts working in industry.




Somatic Embryogenesis


Book Description

Somatic embryogenesis, the initiation of embryos from previously differentiated somatic cells, is a unique process in plants. This volume expands our view of a subject that is important for plant biotechnology, genetics, cell biology, development, and agricultural applications. All chapters present the latest research progress, including functional genomic, genetic, and proteomic approaches. A special focus is placed on the effects of stress, environment, and plant growth regulators on embryogenesis. The role of genes such as Leafy Cotyledons and Baby Boom in defining and maintaining cell competence is discussed.




Modern Applications of Plant Biotechnology in Pharmaceutical Sciences


Book Description

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions




In Vitro Embryogenesis in Plants


Book Description

In vitro Embryogenesis in Plants is the first book devoted exclusively to this topic. As the ultimate demonstration of totipotency in plants, somatic and haploid embryogenesis is of vital importance to all those working on or interested in basic and applied aspects of plantlet information and regeneration. The text includes comprehensive reviews written by experts, on all facts of in vitro and in vivo embryogenesis. Some chapters deal with the morphogenic, structural and developmental, physiological and biochemical, and molecular biological aspects of the subject. Chapters are also devoted to haploid embryogenesis, asexual embryogenesis in nature, zygotic embryogenesis, and zygotic embryo culture. Detailed tables summarizing successful somatic embryogenesis in all vascular plants are also included. This book, therefore, brings together previously scattered information to provide an indispensable reference book for both active researchers, graduate students and anyone interested in this aspect of tissue culture technology and plant development.




Somatic Embryogenesis and Synthetic Seed I


Book Description

While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro propagation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.




Epigenetics in Plants of Agronomic Importance: Fundamentals and Applications


Book Description

Over the past decades, chromatin remodelling has emerged as an important regulator of gene expression and plant defense. This book provides a detailed understanding of the epigenetic mechanisms involved in plants of agronomic importance. The information presented here is significant because it is expected to provide the knowledge needed to develop in the future treatments to manipulate and selectively activate/inhibit proteins and metabolic pathways to counter pathogens, to treat important diseases and to increase crop productivity. New approaches of this kind and the development of new technologies will certainly increase our knowledge of currently known post-translational modifications and facilitate the understanding of their roles in, for example, host-pathogen interactions and crop productivity. Furthermore, we provide important insight on how the plant epigenome changes in response to developmental or environmental stimuli, how chromatin modifications are established and maintained, to which degree they are used throughout the genome, and how chromatin modifications influence each another.




Plant Embryogenesis


Book Description

Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips. Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism. Provides the most comprehensive collection of protocols on plan embryo development.




Somatic Embryogenesis and Gene Expression


Book Description

Provides an insight into current advances in the development of somatic embryogenesis, as well as the cellular and molecular mechanism, differential gene expression, and extracellular protein markers during different developing stages of somatic embryogenesis. It cover somatic embryogenesis in dicotyledons and monocotyledons plants, different factors regulating somatic embryogenesis (plant genotype, level of sugar in the medium, type and concentration of growth regulators, photoperiod, gelling agents, time exposure, induction and maturation medium), application of complex organic additives (often involves fruit juices, pulp, extracts and homogenates as well as liquid endosperms, protein hydrolysates like casein hydrolysate, tryptone, and yeast lysate like yeast extract), genetic fidelity of somatic embryos derived plantlets and role of connecting stress leading in expression of transcription factor that appears to connect stress and developmental signalling pathways and is essential for the subsequent signal transduction cascade that leads to the formation of somatic embryos. Expression of different genes associated with the accumulation of storage reserve during somatic embryogenesis along with isolation and molecular characterization of miRNAs at different developmental stages of embryogenesis, and finally proteomics during somatic embryogenesis has been discussed in detail.




Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants


Book Description

World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.




Heritable Human Genome Editing


Book Description

Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight.