Fluorescence-Based Biosensors


Book Description

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening. - Contributions from leading authorities - Informs and updates on all the latest developments in the field




Biomedical Photonics Handbook


Book Description

Shaped by Quantum Theory, Technology, and the Genomics RevolutionThe integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in




Biomedical Photonics Handbook, Second Edition


Book Description

Shaped by Quantum Theory, Technology, and the Genomics Revolution The integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in medical diagnostics and therapy. The second edition of the Biomedical Photonics Handbook presents recent fundamental developments as well as important applications of biomedical photonics of interest to scientists, engineers, manufacturers, teachers, students, and clinical providers. The third volume, Therapeutics and Advanced Biophotonics, focuses on therapeutic modalities, advanced biophotonic technologies, and future trends. Represents the Collective Work of over 150 Scientists, Engineers, and Clinicians Designed to display the most recent advances in instrumentation and methods, as well as clinical applications in important areas of biomedical photonics to a broad audience, this three-volume handbook provides an inclusive forum that serves as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of medical technologies. What’s New in This Edition: A wide variety of photonic biochemical sensing technologies has already been developed for clinical monitoring of early disease states and physiological parameters, such as blood pressure, blood chemistry, pH, temperature, and the presence of pathological organisms or biochemical species of clinical importance. Advanced photonic detection technologies integrating the latest knowledge of genomics, proteomics, and metabolomics allow sensing of early disease states, thus revolutionizing the medicine of the future. Nanobiotechnology has opened new possibilities for detection of biomarkers of disease, imaging single molecules and in situ diagnostics at the single-cell level. In addition to these state-of-the-art advancements, the second edition contains new topics and chapters including: • Fiber Optic Probe Design • Laser and Optical Radiation Safety • Photothermal Detection • Multidimensional Fluorescence Imaging • Surface Plasmon Resonance Imaging • Molecular Contrast Optical Coherence Tomography • Multiscale Photoacoustics • Polarized Light for Medical Diagnostics • Quantitative Diffuse Reflectance Imaging • Interferometric Light Scattering • Nonlinear Interferometric Vibrational Imaging • Nanoscintillator-Based Therapy • SERS Molecular Sentinel Nanoprobes • Plasmonic Coupling Interference Nanoprobes Comprised of three books: Volume I: Fundamentals, Devices, and Techniques; Volume II: Biomedical Diagnostics; and Volume III: Therapeutics and Advanced Biophotonics, this second edition contains eight sections, and provides introductory material in each chapter. It also includes an overview of the topic, an extensive collection of spectroscopic data, and a list of references for further reading.




Near-Infrared Dyes for High Technology Applications


Book Description

This book summarizes the results of the NATO Advanced Research Workshop on "Syntheses, Optical Properties, and Applications of Near-Infrared (NIR) Dyes in High Technology Fields" held at Trest (Czech Republic) from September 24 to 27, 1997. It was attended by 52 scientists from 16 countries, among them 18 participants from 8 Eastern-Europe. The Workshop has been stimulated by the great progress that has been made in utilizing NIR dyes in high technology areas. This has been triggered by the availability of reliable. vel)' small, and inexpensive laser diodes emitting in the NIR region which raise the analytical detection limits by several orders of magnitude, allow time-resolved measurements for temporal discrimination between spectrally similar species, and make possible miniaturization as well as remote sensing through space and by fiber optical techniques. Furthermore, NIR dyes proved to be vel)' important in other high technology fields such as laser physics, optical recording techniques, nonlinear optics, biochemistty and medicine.




Contrast Agents II


Book Description

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Molecular Imaging


Book Description

The field of molecular imaging of living subjects have evolved considerably and have seen spectacular advances in chemistry, engineering and biomedical applications. This textbook was designed to fill the need for an authoritative source for this multi-disciplinary field. We have been fortunate to recruit over 80 leading authors contributing 75 individual chapters. Given the multidisciplinary nature of the field, the book is broken into six different sections: "Molecular Imaging technologies", "Chemistry", "Molecular Imaging in Cell and Molecular Biology", "Applications of Molecular Imaging", "Molecular Imaging in Drug Evaluation" with the final section comprised of chapters on computation, bioinformatics and modeling. The organization of this large amount of information is logical and strives to avoid redundancies among chapters. It encourages the use of figures to illustrate concepts and to provide numerous molecular imaging examples.




Fluorescent Dye Labels and Stains


Book Description

Fluorescent Dye Labels and Stains The only comprehensive database of fluorophores and their physical and photochemical properties Fluorophores are chemical compounds that strongly absorb in the ultraviolet, visible, and/or near-infrared and with bright emission in these ranges. As a result, they are exceptionally valuable as dyes for various analytical processes, capable of labelling and staining particular targets for purposes of fluorescent imaging, sensitive detection, and quantification (exhibiting linear responses over very wide concentration ranges). These compounds are many and varied, and panoramic views of their options, physical properties and their reactions to light excitations can be critical to their successful integration into chemical analysis, pharmaceutical analysis, clinical analysis, microscopies, optical bioimaging, cancer imaging, real-time PCR, flow cytometry, multiplexing in proteomics, life sciences in general, and many other high-tech fields (material sciences, traceability, photovoltaics, quantum computing). Fluorescent Dye Labels and Stains incorporates a comprehensive database of such substances and their characteristics. It provides an introduction to basic theories and foundational terminology, in addition to both the molecular structures and photophysical properties of an enormous range of fluorophores. Assembled over the course of a distinguished career in biochemistry, this database presents valuable information that has never before been available in a single volume. Readers will also find: Molecular and photochemical information of over 700 fluorophores A database of parameters, including light excitation ranges, molar absorption coefficients, fluorescence quantum yields, molecular brightness, and many more Information derived from multiple disciplines, including microscopy, nanoscopy, biochemistry, and molecular biology Fluorescent Dye Labels and Stains is the essential reference for pharmaceutical and biomedical researchers and professionals, academics who study molecular biology or organic chemistry, and any professional whose work includes strong and photostable molecular absorptions and fluorescence.




Molecular Imaging in Oncology


Book Description

With molecular imaging becoming one the fastest growing topics in medical schools, Informa Healthcare presents Molecular Imaging in Oncology, the first comprehensive reference on molecular imaging in oncology.Giving clinicians and researchers a greater understanding of the current field, this text covers:instrumentation and techniquescancer imaging




Molecular Imaging Probes For Cancer Research


Book Description

This review volume integrates the advances in cancer biology, molecular imaging techniques and imaging probes for visualization and quantitative measurement of anatomical, functional, and molecular profiles of cancer. The volume also presents a comprehensive summary of the state-of-the-art technology in molecular imaging probe design and applications in radionuclide (PET and SPECT), magnetic resonance (MR), optical (fluorescence, Raman, photoacoustic), ultrasound, CT, and multimodality imaging. Bringing together the fundamentals of molecular imaging, and the basic principles of each molecular imaging modality in this volume, readers' understanding in this field is further enhanced. With a strong emphasis on the chemistry of the design of appropriate molecular imaging probes for early cancer detection, therapy-response monitoring, and anti-cancer drug development, the process of translating novel cancer imaging probes from bench to bedside is extensively discussed.




Textbook of in vivo Imaging in Vertebrates


Book Description

This book describes the new imaging techniques being developed to monitor physiological, cellular and subcellular function within living animals. This exciting field of imaging science brings together physics, chemistry, engineering, biology and medicine to yield powerful and versatile imaging approaches. By combining advanced non-invasive imaging technologies with new mechanisms for visualizing biochemical events and protein and gene function, non-invasive vertebrate imaging enables the in vivo study of biology and offers rapid routes from basic discovery to drug development and clinical application. Combined with the availability of an increasing number of animal models of human disease, and the ability to perform longitudinal studies of disease evolution and of the long-term effects of therapeutic procedures, this new technology offers the next generation of tools for biomedical research. Well illustrated, largely in colour, the book reviews the most common and technologically advanced methods for vertebrate imaging, presented in a clear, comprehensive format. The basic principles are described, followed by several examples of the use of imaging in the study of living multicellular organisms, concentrating on small animal models of human diseases. The book illustrates: The types of information that can be obtained with modern in vivo imaging; The substitution of imaging methods for more destructive histological techniques; The advantages conferred by in vivo imaging in building a more accurate picture of the response of tissues to stimuli over time while significantly reducing the number of animals required for such studies. Part 1 describes current techniques in in vivo imaging, providing specialists and laboratory scientists from all disciplines with clear and helpful information regarding the tools available for their specific research field. Part 2 looks in more detail at imaging organ development and function, covering the brain, heart, lung and others. Part 3 describes the use of imaging to monitor various new types of therapy, following the reaction in an individual organism over time, e.g. after gene or cell therapy. Most chapters are written by teams of physicists and biologists, giving a balanced coherent description of each technique and its potential applications.