The Cytochemical Bioassay of Polypeptide Hormones


Book Description

The cytochemical bioassay system was described in a short abstract in 1971, and more fully, in the cytochemical bioassay of corticotrophin, in 1972. Since then, cytochemical bioassays have been described for several polypeptide hormones, and these assays are already widely used. It is expedient that the subject should be reviewed, as it is in this monograph, by one writer who has had the good fortune to have taken part in the growth of cytochemistry from its early origins to its present position as the basis of possibly the most sensitive bioassay system currently available. However, it should be noted that major contributions have been made by many, both to the development of the subject and to the establishment of the bioassays. The object of this preface is to try to give some perspective to the growth of this subject and to record that the cytochemical bioassay system has been fostered by many outstanding scientists in an atmosphere of remarkable goodwill. To begin with, there could have been no cytochemical bioassays until cytochem istry had been converted from its rather unsure origins into a precise and quantitative form of cellular biochemistry. This was done with skill and enthusiastic dedication by my colleagues, Dr. Lucille Bitensky, Dr. F. P. Altman, Dr. R. G. L. W. Poulter and Mr. A. A. Silcox, first at the Royal College of Butcher, Dr.




Cytochemical Bioassays


Book Description

Cytochemical Bioassays: Techniques and Clinical Applications describes the techniques and clinical applications of cytochemical bioassays, particularly with respect to polypeptide hormones. This book discusses the clinical and research implications of studies that use these techniques. This text is comprised of 14 chapters; the first of which introduces the reader to the origins and general principles of cytochemical bioassays, along with their advantages. This discussion is followed by a chapter that explains what is meant by ""an assay""; for what purpose assays are done; the basic components of an assay method; and the nature of the difference between in vivo and in vitro hormone bioassays, in vitro ligand assays, and enzyme assays. Attention then turns to the techniques of cytochemical bioassays and the cytochemical bioassay procedures for adrenocorticotropic hormones; thyroid-stimulating hormones; the thyroid-stimulating antibody of Graves' disease; thyroid growth stimulating and blocking immunoglobulins; and luteinizing hormones. The next chapters focus on the technique and clinical relevance of a cytochemical bioassay for gastrin-like activity and for measurement of antidiuretic hormones, parathyroid hormones, angiotensin II, natriuretic hormones, and hypothalamic regulating hormones. This book will be of interest to clinicians and practitioners of biology, biochemistry, and endocrinology.




Peptide Hormone Secretion


Book Description

Polypeptide hormones play a central role in the regulation of biological processes from the minute-to-minute control of vital functions to the programming of birth, development, reproduction, and ageing. The first of a two-volume work on the methods of study used in this field, Peptide Hormone Secretion focuses on the isolation of polypeptide hormones and their identification and structural analysis, the development of sensitive bioassay and immunoassay techniques, and tissue localization of peptide hormones and their encoding MRNAs, as well as techniques for characterization of peptide hormone receptors. Ideal for graduate students and researchers, the book includes coverage of the general principles and strategies that the methods are based on, so the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation.




Theory and Strategy in Histochemistry


Book Description

If you want practical information on how to use this book please refer to "Note to the Readers" p. VII. Histochemistry and cytochemistry are essential tools in biomedical research and routine service laboratories. Most texts on histochemistry fall into one of two categories: 1. Encyclopaedic texts covering all or nearly all information available on the whole or selected parts of histochemistry. 2. Reviews or surveys of methods found to be useful by the author(s). While the former category often appeals to the more philosophically inclined reader, direct guidance on the selection of technique may be difficult to find. In contrast, the latter category are often excellent sources for details on how to perform a particular method with a reasonable chance of success. Consideration of the exact mechanism of staining, of possible reasons for failure, and of alternative techniques are, however, frequently lacking. This book is an introduction to the scientific basis of histochemistry and is intended to provide a background for the selection and development of appro priate methods. It is not a "cook book" and readers expecting exhaustive methodological descriptions will be disappointed. Although most ofthe contributors to this book would not describe themselves as histochemists, they have all at some time found it essential to develop a basic understanding of histochemistry. This book contains the information they would have greatly appreciated ready access to at that time.










Current Catalog


Book Description

First multi-year cumulation covers six years: 1965-70.




Endocrine Disorders


Book Description

Endocrine Disorders







Amino Acids, Peptides and Proteins


Book Description

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.