The Forensic Value of Processed Human Hair Extensions


Book Description

The human hair extension industry has grown immensely with revenues exceeding nine billion dollars each year. Statistics indicate that over sixty percent of women have at some point invested in hair extensions and that they are even becoming popular with men. In spite of the expansion of the extension consumer market, human hair extensions have never been evaluated for their evidentiary value in a forensic case. A human hair extension collected from a crime scene would be evaluated as a shed human telogen hair and analyze during microscopic techniques and mitochondrial DNA (mtDNA). Sequencing of mtDNA from the extension would place the hair donor, not the suspect, at the scene. Although it is not likely that the mtDNA sequence would be matched to the donor’s maternal lineage, the evidence would be misleading. Hair extensions collected from a crime scene would misdirect an investigation and result in a misuse of time and resources. The ability to identify a human hair as an extension would be invaluable during an investigation and might exclude the hair as probative evidence. The human hair extension industry has grown immensely with revenues exceeding nine billion dollars each year. Statistics indicate that over sixty percent of women have at some point invested in hair extensions and that they are even becoming popular with men. In spite of the expansion of the extension consumer market, human hair extensions have never been evaluated for their evidentiary value in a forensic case. A human hair extension collected from a crime scene would be evaluated as a shed human telogen hair and analyze during microscopic techniques and mitochondrial DNA (mtDNA). Sequencing of mtDNA from the extension would place the hair donor, not the suspect, at the scene. Although it is not likely that the mtDNA sequence would be matched to the donor’s maternal lineage, the evidence would be misleading. Hair extensions collected from a crime scene would misdirect an investigation and result in a misuse of time and resources. The ability to identify a human hair as an extension would be invaluable during an investigation and might exclude the hair as probative evidence. mtDNA extracted from the hair extensions was sequenced and compared to the revised Cambridge sequence (rCRS) to identify single nucleotide polymorphisms (SNPs). SNPs were used to assign haplotypes and distinguish regional affiliations associated with the extensions in an attempt to establish the ethnicity of the hair donor’s maternal lineage. Haplotype assignments for the hair extensions were based on HV2 genetic polymorphisms and represented multiple geographic regions and a large portion of the population. HV2 sequences were not restrictive enough to determine regional affiliations for particular extension brands or processed human hair extensions as a whole. More definitive haplotype assignments would be possible with HV1 SNP discrimination. Also, sequence variation between hair extensions of the same brand indicated that the hair within a single package of extensions was from multiple donors. This has significant implications in forensic analysis.




Chemical Analysis of Proecessed Human Hair Extentions for Use in Forensic Casework


Book Description

In forensic laboratories, hair analysis is performed in the trace evidence unit and the DNA unit. The completed analysis of hair can include a variety of tests, but generally, microscopic analysis is performed and is followed by mitochondrial DNA analysis. With the increasing use of hair extensions, it is possible that a hair sample discovered at a crime scene may be a processed human hair extension and has no physical or genetic connection to the individual wearing the hair extensions. This can be misleading in an investigation and result in a misuse of time and resources. Being able to identify a hair as a processed human hair extension is valuable in determining its entire evidentiary value and result in the forensic laboratory adjusting its approach to analysis. Current literature is limited pertaining to hair extensions as forensic evidence. Only one study has focused extensively on processed human hair extensions for their probative value in forensic case work. This study involved the microscopic examination and genetic analysis of processed human hair extensions but found no features that differentiated natural human head hairs from human hair extensions. There are multiple articles that research chemical analysis of hair, but few are looking into applications into the forensic laboratory. The lack of literature proves the necessity for this research.This research determined whether a chemical difference was present between natural human head hair and processed human hair extensions through ultraviolet-visible spectroscopy, gas chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy. Three different hair extension brands and five different natural human hair samples were used in this research. Multiple organic solid-liquid extractions were completed using hexanes, Colorist SecretsTM Hair Color Remover, methanol, 1-octanol, and chloroform-d. The extraction solvents were then used for instrumental analysis by ultraviolet-visible spectroscopy, gas chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy. The resulting spectra were analyzed and compared to determine differences between processed human hair extensions and natural human head hairs. This study proves that there is a chemical difference between processed human hair extensions and natural human head hair. This was indicated by the differences observed in spectra on the UV-Vis, GC/MS, and NMR between the natural human head hairs and processed human hair extensions. The processed human hair extensions have a higher absorbance, when analyzed with UV-Vis spectroscopy than the natural human head hair samples. In addition, there are specifics in the processed human hair extensions that are visualized by GC/MS and NMR analysis that are not present in the control natural human head hair samples. The higher absorbance and peaks show a chemical difference between the samples. This chemical difference can be used to differentiate samples in a forensic laboratory, which could change how hair is analyzed for casework. This research is significant because it allows for the chemical identification of processed human hair extensions which may help in determining if the hair has probative value in a forensic case. The ability to chemically identify a hair extension collected from a crime scene would provide investigate leads and help to preserve time and resources. Also, chemical analysis might allow a processed human hair extension collected from a crime scene to be definitively associated with others from the same manufacturing package. This would be beneficial if the extensions were still attached to the suspect's head. This research has the potential to change the way that hair analysis is viewed and used throughout the forensic laboratory. Further research needs to be completed: analysis of more hair extension brands, determine a concise method of analysis, and the best extraction solvent. The study is a precursor to all of this and proves that there is a necessity for further analysis and implementation to forensics. But before any of that can happen, a reliable and consistent method needs to be determined and validated. This study proves this need for further research in this area, and ultimately updating the process for hair analysis throughout forensic laboratories.




Chemical and Physical Behavior of Human Hair


Book Description

Human hair is the subject of a wide range of scientific investigations. Its chemical and physical properties are of importance to the cosmetics industry, forensic scientists, and to biomedical researchers. This updated and enlarged fourth edition continues the tradition of its predecessor as being the definitive monograph on the subject. It now contains new information on various topics including: chemical hair damage, the cause of dandruff, skin and eye irritation, hair straightening, and others. Chemical and Physical Behavior of Human Hair is a teaching guide and reference volume for cosmetic chemists and other scientists in the hair products industry, academic researchers studying hair and hair growth, textile scientists, and forensic specialists.




Forensic DNA Typing Protocols


Book Description

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.




Forensic DNA Analysis


Book Description

The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing, forensic casework, family lineage studies, identification of human remains, and DNA databasing. Forensic DNA Analysis:




Forensic DNA Evidence Interpretation


Book Description

Now in its second edition, Forensic DNA Evidence Interpretation is the most comprehensive resource for DNA casework available today. Written by leaders in the fields of biology and statistics, including a contribution from Peter Gill, the father of DNA analysis, the book emphasizes the interpretation of test results and provides the necessary formulae in an easily accessible manner. This latest edition is fully updated and includes current and emerging techniques in this fast-moving field. The book begins by reviewing all pertinent biology, and then provides information on every aspect of DNA analysis. This includes modern interpretation methods and contemporary population genetic models available for estimating DNA frequencies or likelihood ratios. Following a chapter on procedures for validating databases, the text presents overviews and performance assessments of both modern sampling uncertainty methods and current paternity testing techniques, including new guidelines on paternity testing in alignment with the International Society for Forensic Genetics. Later chapters discuss the latest methods for mixture analysis, LCN (ultra trace) analysis and non-autosomal (mito, X, and Y) DNA analysis. The text concludes with an overview of procedures for disaster victim identification and information on DNA intelligence databases. Highlights of the second edition include: New information about PCR processes, heterozygote balance and back and forward stuttering New information on the interpretation of low template DNA, drop models and continuous models Additional coverage of lineage marker subpopulation effects, mixtures and combinations with autosomal markers This authoritative book provides a link among the biological, forensic, and interpretative domains of the DNA profiling field. It continues to serve as an invaluable resource that allows forensic scientists, technicians, molecular biologists and attorneys to use forensic DNA evidence to its greatest potential.







Strengthening Forensic Science in the United States


Book Description

Scores of talented and dedicated people serve the forensic science community, performing vitally important work. However, they are often constrained by lack of adequate resources, sound policies, and national support. It is clear that change and advancements, both systematic and scientific, are needed in a number of forensic science disciplines to ensure the reliability of work, establish enforceable standards, and promote best practices with consistent application. Strengthening Forensic Science in the United States: A Path Forward provides a detailed plan for addressing these needs and suggests the creation of a new government entity, the National Institute of Forensic Science, to establish and enforce standards within the forensic science community. The benefits of improving and regulating the forensic science disciplines are clear: assisting law enforcement officials, enhancing homeland security, and reducing the risk of wrongful conviction and exoneration. Strengthening Forensic Science in the United States gives a full account of what is needed to advance the forensic science disciplines, including upgrading of systems and organizational structures, better training, widespread adoption of uniform and enforceable best practices, and mandatory certification and accreditation programs. While this book provides an essential call-to-action for congress and policy makers, it also serves as a vital tool for law enforcement agencies, criminal prosecutors and attorneys, and forensic science educators.







DNA Technology in Forensic Science


Book Description

Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.