The Interaction of Pasteurella Multocida Toxin with Mammalian Cells
Author : Michael Robert Baldwin
Publisher :
Page : 476 pages
File Size : 20,23 MB
Release : 2003
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ISBN :
Author : Michael Robert Baldwin
Publisher :
Page : 476 pages
File Size : 20,23 MB
Release : 2003
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Author : Klaus Aktories
Publisher : Springer Science & Business Media
Page : 153 pages
File Size : 11,82 MB
Release : 2012-09-03
Category : Medical
ISBN : 3642310176
This volume brings together contributions from experts in the field of Pasteurella research. Its covers areas such as comparative genomics, pathogenic mechanisms, bacterial proteomics, as well as a detailed description and analysis of PMT and its interaction with host tissues, cells, immune system, and signalling pathways.
Author : Klaus Aktories
Publisher : Springer
Page : 150 pages
File Size : 11,34 MB
Release : 2012-09-04
Category : Medical
ISBN : 9783642310188
This volume brings together contributions from experts in the field of Pasteurella research. Its covers areas such as comparative genomics, pathogenic mechanisms, bacterial proteomics, as well as a detailed description and analysis of PMT and its interaction with host tissues, cells, immune system, and signalling pathways.
Author : Alistair J. Lax
Publisher : Cambridge University Press
Page : 268 pages
File Size : 42,20 MB
Release : 2011-04-28
Category : Science
ISBN : 9781139443593
Bacterial toxins that act inside cells interact very specifically with key components of the cell and some even manipulate the cell in subtle ways for their own purposes. These potent toxins, described in this 2005 book, will be of interest to both microbiologists and cell biologists. Some of these toxins are conventional multidomain toxins that are self-programmed to enter cells. Others are delivered by type III mechanisms, often as a package of potent molecules. The molecular targets for all these toxins mediate signal transduction and the cell cycle to regulate the crucial processes of cell growth, cell division and differentiation. Thus these potent toxins are not only responsible for disease, but also provide a powerful set of tools with which to interrogate the biology of the cell. In addition such toxins may act directly to promote carcinogenesis and hence their study is also of interest in a wider context.
Author :
Publisher :
Page : pages
File Size : 34,25 MB
Release : 2014
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Author : John William Peterson
Publisher :
Page : 458 pages
File Size : 12,77 MB
Release : 2003
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Author : Rebecca Claire Babb
Publisher :
Page : 388 pages
File Size : 50,57 MB
Release : 2010
Category : Bacterial toxins
ISBN :
Many bacterial toxins covalently modify components of eukaryotic signalling pathways in a highly specific manner, and can be used as powerful tools to decipher the function of their molecular target(s). The Pasteurella multocida toxin (PMT) mediates its cellular effects through the activation of three of the four heterotrimeric G-protein families, Gq, G12 and Gj. -- I have investigated modification of Ga subunits mediated by PMT in vivo using 2-D gel electrophoresis and antibody detection. A change in the isoelectric point was observed for the Ga subunit of the Gq and Gi families, but not G5 , following PMT treatment of Swiss 3T3 cells. PMT induced the modification of Ga11 which is not activated by PMT. I suggest that the modification of these G-proteins is due to tyrosine phosphorylation. Ga13 is also modified by PMT treatment. Prolonged treatment with PMT led to a reduction in membrane-associated Gai, but not Gaq. I also show that PMT inhibits the GTPase activity of Gq using a trypsin protection assay that probes the conformation state of a G-protein and demonstrated that PMT inhibits the basal and receptor activated steady state GTPase activity in Swiss 3T3 membranes. -- During the course of my research Gai has recently been shown to be deamidated at Gln205 by PMT to inhibit its intrinsic GTPase activity. My findings support the concept that PMT functions as an archetypal intracellular toxin to induce the covalent modification of G-proteins and to stimulate their activation through a common mechanism.
Author : Mark Allen Waites
Publisher :
Page : 188 pages
File Size : 18,14 MB
Release : 2002
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Author : Julia K. Ebner
Publisher :
Page : pages
File Size : 12,93 MB
Release : 2018
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Author : K. Aktories
Publisher : Springer Science & Business Media
Page : 713 pages
File Size : 48,61 MB
Release : 2013-03-14
Category : Medical
ISBN : 3662059711
In recent years remarkable progress has been accomplished with respect to our knowledge about bacterial protein toxins. This refers especially to structural aspects of protein toxins but also holds true for genetics, molecular biology and biochemical mechanisms underlying the action of toxins. This volume covers the very current and exciting aspects of up-to-date bacterial toxicology and comprehensively reviews the most important bacterial protein toxins such as the intracellular acting toxins which exhibit enzyme activity, as well as those toxins that interact with cell plasma membranes by damaging the membranes (pore formation) or stimulating cell receptors (superantigens). This is the most current reference work on these important bacterial protein toxins, which are presented from the point of view of different disciplines such as pharmacology, microbiology, cell biology and protein chemistry.