Techniques In Microscopy For Biomedical Applications


Book Description

The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.




Biomedical Optical Phase Microscopy and Nanoscopy


Book Description

Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.




Biomedical Visualisation


Book Description

This edited volume explores the use of technology to enable us to visualise the life sciences in a more meaningful and engaging way. It will enable those interested in visualisation techniques to gain a better understanding of the applications that can be used in imaging and analysis, education, engagement and training. The reader will be able to explore the utilisation of technologies from a number of fields to enable an engaging and meaningful visual representation of the life sciences. This use of technology-enhanced learning will be of benefit for the learner, trainer, in patient care and the wider field of education and engagement. By examining a range of techniques in image capture (photogrammetery, stereophotogrammetry, microphotogrammetry and autostereoscopy), this book will showcase the wide range of tools we can use. Researchers in this field will be able to find something suitable to apply to their work to enhance user engagement through improved visual means using the technologies we have available to us today. It will highlight the uses of these technologies to examine many aspects of the human body, and enable improved ways to enhance visual and tactile learning, including 3D printing. By demonstrating co-design processes, working directly with the end-stage users (including patients), it will also highlight successes in adopting tools like hand motion tracking rehabilitation for patients with conditions like multiple sclerosis. The book will also discuss the applications of immersive environments including virtual, augmented and mixed reality. The ultimate aim is to show how, by using these tools, we can enhance communication, mobile applications, health literacy and illustration of both normal and pathological processes in the body. By applying a wide range of tools and technologies, this volume will highlight the wide range of applications in education, training and learning both for students and faculty, but also for patient care and education. Therefore, the work presented here can be accessed by a wide range of users from faculty and students involved in the design and development of these processes, by examining the pedagogy around these technologies. Importantly, it presents material, which will be of benefit for the patient, engaging them to become more involved with techniques like physiotherapy.




Biomedical Visualisation


Book Description

This book highlights the integration of science and imaging and demonstrates how we can teach and learn in a much more accessible, innovative, and engaging way using technology. This volume is particularly focused on three main themes: advanced microscopy, anatomy education, and radiology visualisation related to patient care. The chapters pertaining to advanced microscopy convey complex biomedical information by visual means. These chapters provide both an overview on the principles of microscopy and specific applications of microscopy that have led to groundbreaking discoveries. Chapters pertaining to education summarise the recent trends in teaching gross and microscopic anatomy and emphasise the creation and use of novel tools to support student learning. Lastly, the radiological visualisation segment dives into the history of radiographic imaging and highlights the profound effect technology has had on improving patient outcomes. This volume will be of particular interest to many; the scope of this book encompasses medicine, dentistry, allied health professions, biomedical sciences, anatomy and histology education, radiology, and microscopy. Students, researchers, educators, and clinicians will learn something new, be stimulated to ask innovative questions, and be inspired to continue the technological advancements pushing science forward.




Handbook of Biological Confocal Microscopy


Book Description

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.




Biomedical Optical Imaging


Book Description

Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.




Focus on Multidimensional Microscopy


Book Description

This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.




Principles Of Three-dimensional Imaging In Confocal Microscopes


Book Description

This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.




Biomedical Applications of Microprobe Analysis


Book Description

Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter