Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 220 pages
File Size : 27,76 MB
Release : 2007-12-31
Category : Science
ISBN : 0387346082
The Journal of Fluorescence’s fourth Who’s Who directory is to publish the names, contact details, specialty keywords, and a brief description of scientists employing fluorescence methodology and instrumentation in their working lives. In addition, the directory will provide company contact details with a brief list of fluorescence-related products. The directory will be edited by Chris D. Geddes and Joseph R. Lakowicz, editor and founding editor of the Journal of Fluorescence.
Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
Page : 352 pages
File Size : 34,62 MB
Release : 2011-03-29
Category : Science
ISBN : 3642180353
The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.
Author : Kevin F. Sullivan
Publisher : Elsevier
Page : 613 pages
File Size : 16,92 MB
Release : 2007-12-14
Category : Science
ISBN : 0080557244
This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
Author : Chris D. Geddes
Publisher : Springer Science & Business Media
Page : 346 pages
File Size : 25,1 MB
Release : 2005-06-28
Category : Science
ISBN : 9780387233345
Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.
Author : Vincent Pieribone
Publisher : Harvard University Press
Page : 306 pages
File Size : 40,49 MB
Release : 2005
Category : Nature
ISBN : 9780674019218
The discovery of green fluorescent protein revolutionized molecular biology, transforming our study of everything from the AIDS virus to the workings of the brain.
Author : Chris D Geddes
Publisher : Springer Science & Business Media
Page : 402 pages
File Size : 21,97 MB
Release : 2009-11-19
Category : Science
ISBN : 0387887229
This fourth volume in the Springer series summarizes the year’s progress in fluorescence, with authoritative analytical reviews specialized enough for professional researchers, yet also appealing to a wider audience of scientists in related fields.
Author : Ute Resch-Genger
Publisher : Springer Science & Business Media
Page : 566 pages
File Size : 40,90 MB
Release : 2008-08-15
Category : Science
ISBN : 3540705716
Analytical chemists and materials scientists will find this a useful addition to their armory. The contributors have sought to highlight the present state of affairs in the validation and quality assurance of fluorescence measurements, as well as the need for future standards. Methods included range from steady-state fluorometry and microfluorometry, microscopy, and micro-array technology, to time-resolved fluorescence and fluorescence depolarization imaging techniques.
Author : Laura Marcu
Publisher : CRC Press
Page : 554 pages
File Size : 13,19 MB
Release : 2014-07-17
Category : Science
ISBN : 1439861684
During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia
Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
Page : 461 pages
File Size : 33,51 MB
Release : 2010-09-08
Category : Science
ISBN : 3642047017
With contributions by numerous experts
Author : Gregor Jung
Publisher : Springer Science & Business Media
Page : 287 pages
File Size : 34,99 MB
Release : 2012-01-05
Category : Science
ISBN : 3642233767
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
