Author : Oana-Maria Boldura
Publisher : BoD – Books on Demand
Page : 207 pages
File Size : 17,48 MB
Release : 2020-06-24
Category : Science
ISBN : 1789848563
This book explores the role of nucleic acid analysis and the advances it has led to in the field of life sciences. The first section is a collection of chapters covering experimental methods used in molecular biology, the techniques adjacent to these methods, and the steps of analysis before and after obtaining raw DNA data. The second section deals with the principles of chromatography, method development, sample preparation, and industrial applications.
Author : Oana-Maria Boldura
Publisher :
Page : pages
File Size : 36,49 MB
Release : 2020
Category : Analytical biochemistry
ISBN : 9781838809034
Author : Rajan Katoch
Publisher : Springer Science & Business Media
Page : 449 pages
File Size : 41,57 MB
Release : 2011-07-19
Category : Science
ISBN : 1441997857
Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
Author : Saroj Dua
Publisher : Alpha Science International, Limited
Page : 228 pages
File Size : 20,72 MB
Release : 2010
Category : Medical
ISBN :
A comprehensive discussion of the various analytical techniques that are carried out in biochemistry, intends to support students in grasping techniques which are of use for theoretical and practical purposes.
Author : Friedrich Lottspeich
Publisher : John Wiley & Sons
Page : 1151 pages
File Size : 31,72 MB
Release : 2018-05-29
Category : Science
ISBN : 3527339191
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
Author : David Glick
Publisher : John Wiley & Sons
Page : 537 pages
File Size : 36,3 MB
Release : 2009-09-25
Category : Science
ISBN : 0470110899
Biochemical analysis is a rapidly expanding field and is a key component of modern drug discovery and research. Methods of Biochemical Analysis provides a periodic and authoritative review of the latest achievements in biochemical analysis. Founded in 1954 by Professor David Glick, Methods of Biochemical Analysis provides a timely review of the latest developments in the field.
Author : Khursheed Hussain
Publisher : Elsevier
Page : 258 pages
File Size : 30,32 MB
Release : 2024-04-17
Category : Science
ISBN : 0443159157
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids and more. The large range of protocols covered in this foundational how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects. Features step-by-step methods for biochemical analysis of a variety of compounds Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas Provides detailed instructions on how to prepare buffers, equipment to be used and analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones Contains interdisciplinary and adaptable methods and techniques
Author : David Glick
Publisher : John Wiley & Sons
Page : 450 pages
File Size : 26,65 MB
Release : 2009-09-25
Category : Science
ISBN : 0470110945
Biochemical analysis is a rapidly expanding field and is a key component of modern drug discovery and research. Methods of Biochemical Analysis provides a periodic and authoritative review of the latest achievements in biochemical analysis. Founded in 1954 by Professor David Glick, Methods of Biochemical Analysis provides a timely review of the latest developments in the field.
Author : S. Sadasivam
Publisher : New Age International
Page : 276 pages
File Size : 47,41 MB
Release : 1996
Category : Biochemistry
ISBN : 9788122409765
Biochemical Methods Are Used In All Branches Of Biological Sciences And Agriculture Is No Exception. Research In Various Branches Of Agriculture Viz. Plant Physiology, Plant Pathology, Agricultural Microbiology Seed Technology Plant Genetics And Entomology Requires One Or The Other Biochemical Methods. A Researcher Has To Refer Many Journals And Books Before He Could Get To The Right Procedure For His Experiment. This Book On Biochemical Methods Attempts To Give Often Used Methods In A Single VolumeThe Book, Divided Into 13 Chapters Contains 115 Procedures. The Chapters Are Carbohydrates, Lipids, Proteins, Nucleic Acids, Vitamins, Enzymes, Nitrogen Fixation Antinutritional Factors, Plant Hormones, Pigments, Phenols Cell Fractionation And Separation Techniques. Each Procedure Is Divided Into Introduction, Principle, Materials, Procedure And Calculation. At The End Of Each Procedure References For Additional Reading Are Provided. Important Precautions, Warnings And Tips Are Given In The Notes Section.The Methods Elaborated In The Book Will Be Useful For Conducting Practical Classes At The Undergraduate And Postgraduate Levels In Science Colleges And Universities. This Manual Will Be A Bonanza For The Research Workers In Plant Sciences Since It Includes Procedures From The Classical Microkjeldahl Nitrogen Estimation To The Modern Southern Blotting Technique.
Author : K. Schügerl
Publisher : Springer
Page : 286 pages
File Size : 27,40 MB
Release : 2003-07-01
Category : Technology & Engineering
ISBN : 3540457364
This special volume "Tools and Applications of Biochemical Engineering Science" is dedicated to Professor Wolf-Dieter Deckwer on the occasion of his 60th bir- day. It was a great pleasure for me to act together with Professor Karl Schtigerl as volume editor and to present here a collection of 11 outstanding review articles written mainly by former students, associates, colleagues and friends of Wolf- Dieter Deckwer. The title of this special volume well reflects the research interests and sci- tific pursuit of Wolf-Dieter Deckwer during his more than 20 years’ work in the area of biochemical engineering, particularly during the last 15 years when he was the head of the Biochemical Engineering Division of GBF (German Nat- nal Research Center for Biotechnology). He has decisively pushed the devel- ment not only of "software tools" ranging from analytical means and mathe- tical models for monitoring and understanding cellular processes to gene expression systems for designing microorganisms, but also of "hardware tools" such as computer control systems, bioreaction and separation devices for eff- tively producing a variety of bioproducts on semi-production scale. New developments in some of these important tools in biochemical engineering are reviewed in articles included in this volume. Wolf-Dieter Deckwer was among the leading biochemical engineers who timely pointed out the necessity of applying these tools in an integrated manner for bioprocess development. By establishing "Integrated Bioprocess Development" as one of the GBF main - search topics as early as 1990 he also actively promoted this idea.
